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ym1 chi3l3  (R&D Systems)


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    R&D Systems ym1 chi3l3
    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 <t>(CHI3L3),</t> Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 <t>(CHI3L3),</t> another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
    Ym1 Chi3l3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chi3l3+mouse/bio_rxiv__64898__2026__01__07__698165-171-43-46?v=R%26D+Systems
    Average 93 stars, based on 32 article reviews
    ym1 chi3l3 - by Bioz Stars, 2026-07
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    Images

    1) Product Images from "Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation"

    Article Title: Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation

    Journal: bioRxiv

    doi: 10.64898/2026.01.07.698165

    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
    Figure Legend Snippet: (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Techniques Used: Expressing, Activation Assay, Immunofluorescence, Imaging, Marker, Binding Assay

    UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
    Figure Legend Snippet: UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Techniques Used: Isolation, Expressing, Derivative Assay



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    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 <t>(CHI3L3),</t> Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 <t>(CHI3L3),</t> another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
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    rabbit anti mouse ym1 (chi3l3)  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc rabbit anti mouse ym1 (chi3l3)
    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 <t>(CHI3L3),</t> Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 <t>(CHI3L3),</t> another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
    Rabbit Anti Mouse Ym1 (Chi3l3), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chi3l3+mouse/pmc06333780__41467_2018_8140_MOESM2_ESM-29-9-22?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti mouse ym1 (chi3l3) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    rabbit anti mouse ym1 (chi3l3  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc rabbit anti mouse ym1 (chi3l3
    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 <t>(CHI3L3),</t> Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 <t>(CHI3L3),</t> another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.
    Rabbit Anti Mouse Ym1 (Chi3l3, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chi3l3+mouse/pmc06333780__41467_2018_8140_MOESM1_ESM-70-27-31?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti mouse ym1 (chi3l3 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Journal: bioRxiv

    Article Title: Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation

    doi: 10.64898/2026.01.07.698165

    Figure Lengend Snippet: (A) Heatmap displaying scaled expression (z-score) of highly expressed representative genes involved in immune regulation, immune activation (e.g., Pdcd1 ) across four cell types: macrophages (MΦ), T cells, migratory dendritic cells (Mig DCs), and fibroblasts (e.g., Pdpn ). Color scale indicates expression levels. (B) Quantification and network map of predicted ligand-receptor interactions between fibroblasts and immune populations (macrophages, Mig DCs, and T cells) using integrated analysis from CellChat, CellPhoneDB, iCellNet, and the Ramilowsky dataset. The bar graph shows the number of unique ligand-receptor interactions per pairwise combination, with the most extensive communication observed between macrophages and fibroblasts. Analyzed with crossTALK IntEraction Network (TALKIEN) with microglia, neutrophils, and proliferating gene sets excluded. (C) Network plot illustrating specific ligand-receptor pairs involved in fibroblast-immune cell communication. Arrows point from ligand-expressing to receptor-expressing cell types. Notable interactions include Spp1-CD44 , Pdpn-Clec2d , and Thbs1-CD47 . (D) Immunofluorescence imaging of the cribriform plate region in EAE day 3.0 mice showing spatial organization of LYVE-1 + lymphatics (white), PDPN + lymphatics and ECM (red), and CSF1R-GFP + myeloid cells (green). DAPI (blue) marks nuclei. Scale bars: 30 µm. (E) Pathway enrichment analysis of ligand-receptor pairs among macrophages, Mig DCs, T cells, and fibroblasts. Enriched pathways include extracellular matrix organization, integrin-mediated interactions, interleukin 10 signaling. Gene ratio represents the proportion of identified genes contributing to each pathway; dot size reflects the number of genes, and color indicates adjusted p-value. (F) : UMAP projection shows major post contact cell types, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Chil3 (CHI3L3), Arg1 , Spp1 , Ccr2 , Cxcr4 , Csf1r (G) : Volcano plot comparing post-contact vs no-contact cluster 2 macrophages (Highest Arg1 expressing cluster). Analysis reveals post contact macrophage have downregulated MHC-II processing and presentation genes ( H2-DMa , H2-Aa , H2-Ab1 , H2-DMb1 , H2-Eb1 , Cd74 , Ciita ) and elevated expression of Chil3 (CHI3L3), another alternatively activated macrophage marker and ECM binding protein. (H) : Gene ontology enrichment analysis (bottom) demonstrates post contact Macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Article Snippet: The following antibodies were used for immunohistochemistry: Podoplanin PE (eBioscience, Catalog #: 12-5381-80), CD31 Alexa647 (BD Biosciences, Catalog #: 563608), Lyve-1 eFluor660 (Thermo Fisher Scientific, Catalog #: 50-0443-80), MHC II eFluor450 (eBioscience, Catalog #: 48-5321-80), CD11c Alexa488 (Thermo Fisher Scientific, Catalog #: 53-0114-80), YM1 / CHI3L3 (R&D Systems Catalog #: AF2446), Arginase-1 (Invitrogen, Catalog #: PA5-29645), Cleaved Caspase-3 (Abcam, Catalog #: E83-77) .

    Techniques: Expressing, Activation Assay, Immunofluorescence, Imaging, Marker, Binding Assay

    UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Journal: bioRxiv

    Article Title: Cribriform Plate Microenvironment Assembles a Suppressive Myeloid Network during EAE-induced Neuroinflammation

    doi: 10.64898/2026.01.07.698165

    Figure Lengend Snippet: UMAP projection (top left) shows major post contact cell types isolated, including macrophages (purple), microglia (green), neutrophils (blue), T cells (orange), proliferating cells (light blue), migratory dendritic cells (yellow-orange), and fibroblasts (aqua). Feature plots highlight expression of Arg1 , Ly6c2 , Ccr2 , Chil3 (CHI3L3), and Pdpn , identifying infiltrating monocyte-derived, alternatively activated macrophages, distinct from T cells ( Cd3e , Ifng ), DCs ( CCR7 , Cd80 ), microglia ( Tmem119 , P2ry12 ), neutrophils ( Ly6g , Cxcr2 ), and proliferating cells ( Mki67 , Top2a ). Gene ontology enrichment analysis (bottom) demonstrates that these post contact macrophages are enriched for pathways in Fcγ receptor and complement receptor signaling, negative regulation of IL-12 production, homotypic cell-cell adhesion, and hemostasis.

    Article Snippet: The following antibodies were used for immunohistochemistry: Podoplanin PE (eBioscience, Catalog #: 12-5381-80), CD31 Alexa647 (BD Biosciences, Catalog #: 563608), Lyve-1 eFluor660 (Thermo Fisher Scientific, Catalog #: 50-0443-80), MHC II eFluor450 (eBioscience, Catalog #: 48-5321-80), CD11c Alexa488 (Thermo Fisher Scientific, Catalog #: 53-0114-80), YM1 / CHI3L3 (R&D Systems Catalog #: AF2446), Arginase-1 (Invitrogen, Catalog #: PA5-29645), Cleaved Caspase-3 (Abcam, Catalog #: E83-77) .

    Techniques: Isolation, Expressing, Derivative Assay